Figure 1

Construction of the chimeras. The domains Xyn and Glu were connected by overlapping-extension PCR. The genes xyn and glu were amplified using p1/p2 and p3/p4, respectively, and the xyn-glu was created using p1/p4 (Upper). Thereafter, the genes glu and xyn were amplified using p5/p6 and p7/p8, respectively. The glu-xyn was created using p5/p8. The wild Xyn and Glu were amplified using p1/p8 and p5/p4, respectively. The fusion genes appear on the gel as discrete bands at ~ 1.4 kb, with molecular masses of approximately the sum of the contributing gene fragments (Middle). The two chimeras appear on the SDS-PAGE gel as discrete bands at ~ 53 kDa, with molecular masses of approximately the sum of the Xyn, linker peptide, and Glu (Lower). Linker: linker-peptide, M: marker of DNA and protein.