Figure 9
Induction of apoptosis in HL-60 cells by GST-TAT-Apoptin opt . An apoptotic assay of HL-60 cells was performed by flow cytometry after 90 ug/ml of the purified E. coli-expressed recombinant GST-TAT-Apoptinopt protein was co-cultured with HL-60 cell for 24 hours (D). Non-apoptosis controls, (A), (B) and (C), were assayed. These represent respectively HL-60 cells only, HL-60 cells co-cultured with protein buffer and HL-60 cells co-cultured with GST protein. The apoptosis induced by CHX was used as a positive apoptosis control.