Figure 5

Productivities of TAT-Apoptin _opt protein and the growth curves of two recombinant E. coli strains. The productivities of His-TAT-Apoptin_opt (A) and GST-TAT-Apoptin_opt (C) using two E. coli strains, BL21(DE3) and BL21(DE3)pLysS containing either pET-TAT-VP3_opt or pGEX-TAT-VP3_opt, respectively, are shown over a time course after IPTG induction at 37 °C. The growth curves of BL21(DE3) and BL21(DE3)pLysS expressing His-TAT-Apoptin_opt (B) and GST-TAT-Apoptin_opt (D) in LB medium post-induction.