Figure 3

Productivities of TAT-Apoptin protein and the growth curves of the three recombinant E. coli strains. The productivities of His-TAT-Apoptin (A) and GST-TAT-Apoptin (C) for the three E. coli strains, BL21(DE3), BL21(DE3)pLysS, and BL21(DE3)CodonPlus-RP containing pET-TAT-VP3 or pGEX-TAT-VP3 are shown over the time course of cultivation at 37 °C after IPTG induction. The growth curves of BL21(DE3), BL21(DE3)CodonPlus-RP and BL21(DE3)pLysS expressing His-TAT-Apoptin (B) and GST-TAT-Apoptin (D), respectively, in LB medium post-induction.