Lipid production in batch and fed-batch cultures of Rhodosporidium toruloidesfrom 5 and 6 carbon carbohydrates

BMC Biotechnology

Table 1 Comparison of lipid production by R. toruloidesCBS14 at different C/N in batch culture

Carbon	C/N	Lipid production rate (g L⁻¹ h⁻¹)	Yield lipid on carbohydrate (g g⁻¹)	Lipid content (% DW)	Biomass (g L⁻¹)	Time at which maximum lipid produced (h)
Xylose	40	0.03	0.08	24	11	73
	65	0.04	0.11	36	10	85
	100	0.04	0.16	45	8	113
Glucose	40	0.08	0.10	23	9	26
	65	0.10	0.18	50	10	49
	100	0.08	0.20	56	9	66

Lipid production rate, yield and percent dry weight and biomass concentration were determined at the time of maximum total lipid produced. Carbon was provided as 30 g L⁻¹ glucose or xylose and (NH₄)₂SO₄ was provided as nitrogen. Cultures were grown in defined medium at pH 4.0, 30°C, 900 rpm, 1.2 vvm aeration.

ISSN: 1472-6750