Figure 2
From: Use of human amelogenin in molecular encapsulation for the design of pH responsive microparticles
SDS-PAGE analysis of encapsulation experiments with amelogenin (AMG) mixed with BSA (Panel A) or insulin (Ins.) (Panel B). By changing the pH from weakly acidic to neutral the aggregation of amelogenin is induced, which entraps the target molecule. Proteins captured in the aggregates are released upon acidification will end up in the solubilized pellet sample (P). The soluble fraction (S) contains proteins not precipitated or captured by the neutralization. The three subpanels are taken from the same gel. Blue arrowsindicate amelogenin, green arrow indicates BSA, and red arrow indicates insulin.