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Figure 1 | BMC Biotechnology

Figure 1

From: New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein

Figure 1

New cell line development for high antibody-producing mammalian cells. (A) Schematic diagram for the split GFP-based cell screening method. (B) Confocal microscopic images of cells transfected with a GFP-overexpressing vector (pEGFP-C1) or bicistronic vector having GFP fragements and both antibody chains (pNGFP-Light and pCGFP-Heavy). After transfection with these vectors, the cells were cultivated for two days at 30°C. DAPI was used for nucleus staining. Antibody titer was quantified from culture supernatant by ELISA.

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