Table 1 Primers and sequences used in making plasmid constructs
From: Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency
Primer | Sequence (5′ → 3′) |
|---|---|
GEM-T7-STNV | caggcggccgctaatacgactcactataggGAGTAAAGACAGGAAAC |
Luc340R | ctgttgagcaattcacgttc |
STNV-luc | GGGAGTAAAGACAGGAAACTTTACTGACTAACCatggaagacgccaa |
STNV-3′F1 | CCGCTTGAAGTCTTTAATTAAATAC |
STNV-3′F2 | CCAAATTGtaagcttctcgagCCCAGAGGTTCACAATG |
STNV-3′R1 | CCTCTGGGActcgagaagcTTACAATTTGGACTTTCCG |
STNV-3′R2 | CCAGGTATAGTTCTACAgttaacccggg |
TBSV-3′F | gggctcgagGTTTGTGGAGATGAGTGT |
TBSV-3′R | ggggttaacGGGCTGCATTTCTGCAA |
TBSV-5′F | Gacggcggccgctaatacgactcactatagg |
TBSV-5′R | cccgccatggTCGCTTGTTTGTTGGAA |
TMV-3′F | gggcaagcttGGTAGTCAAGATGCATAA |
TMV-3′R | tttcccgggTGGGCCCCTACCGGGGGTAA |