Table 1 Sense (F) and antisense (R) oligonucleotides used for PCR amplifications
Primer | Sequence (5’ – 3’) | Hybridization site |
|---|---|---|
AscI_GI_F | TA GGCGC GCC ATGGTCCGTCCTGTAGAA | 5’ terminus of GUS-intron |
GI_NotI_R | TT GCGGCCGCAGAGGATCCTCATTGTTT | 3’ terminus of GUS-intron |
Rep_F | GCCTGAGTTTTGTGGTTGG | internal to PVX RdRp |
GI_R | GGATAGTCTGCCAGTTCAGTTCG | internal to GUS-intron |
GI_XhoI_R | CCTA CTCGAGCCTCATTGTTTGCCTCCC | 3’ terminus of GUS-intron |
PEBVsgPR_F | AACTCGGTTTGCTGACCTAC | PEBV subgenomic promoter |
TRV3UTR_R | ACCTAAAACTTCAGACACGG | 3’ UTR of TRV RNA-2 |
AgeI-GI_F | TATA ACCGGTCATGGTCCGTCCTGTAGAAACC | 5’ terminus of GUS-intron |
pEAQ_F | AACGTTGTCAGATCGTGCTTCGGCACC | 5’ UTR of pEAQ-HT |
pEAQ_R | CTCCTGTTTAGCAGGTCGTCCCTTCAG | 3’ UTR of pEAQ-HT |