Schematic representation of the vector T-DNA regions. Control elements are illustrated in black boxes and coding sequences are shown in white or blue boxes. Overhead arrows represent duplicated subgenomic coat protein promoters driving expression of the inserted transgene. All vectors contain a backbone sequence derived from pBIN19 except for the PVX vectors, which are based on pGreen0000. 35S, dual enhanced Cauliflower mosaic virus (CaMV) 35S promoter; 35ST, CaMV 35S terminator; TEV 5’, Tobacco etch virus 5’ untranslated region; CPMV 5’, Cowpea mosaic virus 5’ untranslated region modified with mutations A115G and U162C; LB and RB, left and right T-DNA borders; RdRp, RNA-dependent RNA polymerase; TGB, triple gene block; CP, coat protein gene; Rz, self-cleaving ribozyme; NOST, nopaline synthase terminator; MP, movement protein gene; VSPT, soybean vegetative storage protein B 3’ untranslated region; NPTII, expression cassette conferring kanamycin resistance; LIR and SIR, long and short intergenic regions of the Bean yellow dwarf virus genome; C1/C2, Bean yellow dwarf virus open reading frames encoding replication initiation proteins REP and REPA.