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Table 1 Strains & vectors used in this study

From: Construction and transformation of a Thermotoga-E. colishuttle vector

Strain or plasmid Size (bp) Description Reference
T. neapolitana DSM 4359   Isolated from African continental solfataric springs [25]
T. maritima MSB8   Isolated from geothermally heated sea floors in Italy and the Azores [1]
T. sp. RQ7   Isolated from geothermally heated sea floors in Ribeira Quente, the Azores [7]
E. coli    
DH5α   F- endA1 hsdR17 (rk-, mk+) supE44 thi-1 λ- recA1 gyrA96 relA1 deoR Δ(lacZYA-argF)- U169 ϕ80dlacZΔM15 [26]
pUC19 2686 A high-copy number E. coli cloning vector containing portions of pBR322 and M13 mp19 GenBank: L09137
pRQ7 846 Cryptic miniplasmid from T. sp. RQ7 [5]
pKT1 3934 pUC-derived plasmid, containing a kan cassette for thermostable kanamycin selections [13]
pDH1 3517 pRQ7 DNA cloned between BamHI and EcoRI sites of pUC19; Apr This study
pDH10 4762 pRQ7 DNA cloned between EcoRI and XbaI sites of pKT1; Apr; Kanr This study; GenBank: JN813374