Table 1 Strains & vectors used in this study
From: Construction and transformation of a Thermotoga-E. colishuttle vector
Strain or plasmid | Size (bp) | Description | Reference |
|---|---|---|---|
Thermotoga | Â | Â | Â |
T. neapolitana DSM 4359 | Â | Isolated from African continental solfataric springs | [25] |
T. maritima MSB8 | Â | Isolated from geothermally heated sea floors in Italy and the Azores | [1] |
T. sp. RQ7 | Â | Isolated from geothermally heated sea floors in Ribeira Quente, the Azores | [7] |
E. coli | Â | Â | Â |
DH5α |  | F- endA1 hsdR17 (rk-, mk+) supE44 thi-1 λ- recA1 gyrA96 relA1 deoR Δ(lacZYA-argF)- U169 ϕ80dlacZΔM15 | [26] |
Plasmids | Â | Â | Â |
pUC19 | 2686 | A high-copy number E. coli cloning vector containing portions of pBR322 and M13 mp19 | GenBank: L09137 |
pRQ7 | 846 | Cryptic miniplasmid from T. sp. RQ7 | [5] |
pKT1 | 3934 | pUC-derived plasmid, containing a kan cassette for thermostable kanamycin selections | [13] |
pDH1 | 3517 | pRQ7 DNA cloned between BamHI and EcoRI sites of pUC19; Apr | This study |
pDH10 | 4762 | pRQ7 DNA cloned between EcoRI and XbaI sites of pKT1; Apr; Kanr | This study; GenBank: JN813374 |