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Table 2 Percent positivity values of test serum samples

From: Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins

 

Protein for immobilization*

Neospora caninum iscomELISA kit

Sample number

MBP-

NcGRA2

MBP-

NcSRS2

NcSAG1

Co- immobilization

Positive

100

100

100

100

100

Negative

10.0

3.5

10.1

8.3

5.3

1

56.5

48.3

51.5

53.4

27.6

2

47.3

52.5

58.2

53.2

30.0

3

57.0

43.2

22.4

45.3

27.6

4

59.3

37.8

51.0

56.0

39.0

5

36.0

29.5

53.1

39.1

41.7

6

19.0

21.2

28.8

27.6

35.9

7

18.5

13.0

14.8

16.8

20.5

8

92.1

73.0

78.3

81.4

56.6

9

45.2

22.5

30.2

31.5

26.8

10

45.6

42.1

37.8

39.5

50.1

11

53.2

36.8

27.2

40.6

38.4

12

15.3

12.3

10.2

14.3

4.1

13

10.0

3.4

5.3

7.6

6.2

14

12.3

4.8

11.8

10.6

17.5

15

4.1

5.7

2.4

3.9

6.3

16

7.3

4.2

5.6

5.6

10.3

17

4.3

5.0

8.3

6.2

2.3

18

5.6

4.1

5.2

5.0

3.0

19

5.3

4.8

7.2

6.5

5.3

20

7.1

3.7

4.4

5.8

3.2

21

3.2

2.5

6.5

4.2

2.1

22

13.3

9.0

8.9

11.0

2.4

23

9.5

6.4

8.2

7.8

2.9

24

6.6

10.2

8.1

8.8

2.1

25

4.5

11.4

4.7

5.8

3.3

26

5.1

8.3

6.0

7.0

2.7

27

5.2

4.7

3.8

4.0

3.0

28

8.0

4.3

4.8

5.1

3.4

29

9.3

5.9

7.3

7.4

3.6

30

6.2

3.6

4.2

4.7

4.7

  1. *The concentrations of MBP-NcGRA2, MBP-NcSRS2 and NcSAG1 were 1.0, 0.5, and 0.25 μg/ml for both individual and co-immobilization.