Evaluation of cattle serum samples with an ELISA. MBP-NcGRA2 (1 μg/ml), MBP-NcSRS2 (0.5 μg/ml), and NcSAG1 (0.25 μg/ml) were co-immobilized on a 96-well microplate. Anti-bovine IgG-HRP was used as the secondary antibody. (A) The ELISA results with the new method developed in this study (n = 3). (B) Diagnostic results with a commercial ELISA kit and © those with this method “+”and “–” represent positive and negative results, respectively.