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Figure 4 | BMC Biotechnology

Figure 4

From: Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins

Figure 4

Evaluation of cattle serum samples with an ELISA. MBP-NcGRA2 (1 μg/ml), MBP-NcSRS2 (0.5 μg/ml), and NcSAG1 (0.25 μg/ml) were co-immobilized on a 96-well microplate. Anti-bovine IgG-HRP was used as the secondary antibody. (A) The ELISA results with the new method developed in this study (n = 3). (B) Diagnostic results with a commercial ELISA kit and © those with this method “+”and “–” represent positive and negative results, respectively.

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