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Figure 5 | BMC Biotechnology

Figure 5

From: Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data

Figure 5

Snapshots of AFST user interfaces. a: The main interface allows users to upload their sequences, specify relevant information about vector and adapter/linker sequences, initiate data processing, and obtain tabular results showing abnormality. b: Details of a normal sequence. The high-quality region between 5TNS-4 (from 2 to 62, marked with blue and green) and 3TNS (from 900 to 926, marked with pink, yellow and blue) is the final clean sequence (i.e., the region with a light red background). The color legends and their meanings can be found by clicking ‘color table’. c: Details of an abnormal sequence. This sequence has RECA abnormality (RECA-Type A1), where the double-stranded cDNA insert is inverted in its orientation and inserted into the double-strand plasmid vector after enzyme digestion. The vector sequence region between 5TNS-2 (highlighted with blue and brown) and 5TSS-1 (highlighted with yellow and pink) is the part that should have been cut off theoretically after enzyme digestion.

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