Figure 3

Detailed illustration of two sub-categories of Type A Restriction Enzyme Cutting Abnormality (RECA-Type A). RECA-Type A indicates that EcoRI site of the vector is cut off whereas XhoI site is kept. A1 is the special case where cDNA is inserted with inversion while cDNA is inserted without inversion for A2. Because XhoI and EcoRI sticky ends cannot be smoothly ligated, so a random sequence fragment between the vector and cDNA end have been detected. Blue stands for the plasmid vector, yellow for EcoRI, brown for XhoI, red for cDNA, gray for a random sequence fragment, pink for Adapter1, and green either for poly(A) in sense strand of cDNA or for poly(T) in non-sense strand of cDNA.