Figure 5

Effect of varying total DNA concentration on CaMV 35 S-p LAMP-BART assay kinetics and reproducibility. Graph of average Tmax values obtained for a constant target copy number present in a range of total maize genomic DNA concentrations, for each of 4 target copy numbers: 300 copies/assay (black circle symbol); 200 copies/assay (red circle symbol); 100 copies/assay (green circle symbol) and 50 copies/assay (blue circle symbol). Errors represent standard deviation of the mean (three replicates). The least variability in Tmax data was observed between the concentrations of genome marked with an arrow (50-500 ng).