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Figure 1 | BMC Biotechnology

Figure 1

From: GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use

Figure 1

Chemistry of the BART bioluminescent coupled assay. PPi liberated during DNA synthesis reacts with adenosine-5'-O-persulfate (APS) in a reaction catalysed by adenosine triphosphate sulfurylase, to form adenosine triphosphate (ATP). A recombinant thermostable firefly luciferase liberates light, CO2, PPi and adenosine monophosphate (AMP) in the presence of the substrates luciferin and oxygen.

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BMC Biotechnology

ISSN: 1472-6750

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