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Table 2 Effect of fluorescence labeling of Sepabeads on CCA immobilization

From: Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts

Labeling Offered protein
(mg/g dry carrier)
Bound protein
(mg/g dry carrier)
Specific activity of bound CCAa (U/mg)
Fluorescein 85 68 1.66 ± 0.06
Fluorescein + Ru(dpp) 85 68 1.50 ± 0.09
None 82 64 1.74 ± 0.17
  1. a Specific activity was measured using the spectrophotometric assay described in Boniello et al. [21]. Experimental conditions were: 40 mM cephalosporin C; 100 mM PPB (pH 8.0); 37°C
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