Figure 7From: Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalystsTime course of internal pH during conversion of cephalosporin C by immobilized CCA. Panel A. Internal pH measurements from four independent experiments. The bulk pH was constant at 8.0. Panel B. Formation of 7-ACA, determined indirectly from the amount of NaOH consumed for pH control. Experimental conditions were 0.65 mg/mL immobilized CCA; 50 mM cephalosporin C; 50 mM PPB (pH 8.0)Back to article page