Figure 6

pH changes in bulk and particle following addition of cephalosporin C, with no pH control (A) or with automated pH control in bulk (B), and spontaneous hydrolysis of cephalosporin C over time (C). Experiments were performed in 50 mM PPB (pH 8.0) at 37°C. The symbols show pH measurements in bulk while the continuous line shows the particle pH. Labeled Sepabeads not containing enzyme were used in these experiments.