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Figure 4 | BMC Biotechnology

Figure 4

From: A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes

Figure 4

SDS-PAGE analysis of recombinant His-EDDIE-AMPs expressed in E. coli BL21 (DE3). Lane M: the molecular weight markers; Lane 1: crude cells extracts of uninduced E. coli BL21 containing pET-His-EDDIE-AMP; Lane 2: crude cells extracts after 5 h past the induction with IPTG of E. coli BL21 containing pET-His-EDDIE-AMP; The molecular weights of the new protein components agree well with those predicted for the fusion proteins.

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