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Figure 3 | BMC Biotechnology

Figure 3

From: Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method

Figure 3

Genomic exicision of positive selection markers using Cre recombinase mRNA. (A) A schematic overview of Cre-mediated excision. Cre recombinase mRNA was microinjected into the gonads of P0 hermaphrodites carrying homozygous vps-45 mini genes flanked by LoxP sites. F1 hermaphrodites were examined by PCR#1 in which Cre-LoxP excision resulted in 410-bp products. Self-progenies (F2 hermaphrodites) were examined by PCR#1 and PCR#2 to confirm that vps-45 mini genes were successfully excised. (B) Frequency of excision (PCR#1 positive/total F1) by microinjection of Cre mRNA. (C) Cre-excision was detected by PCR#1(upper, 410 bp). Unexcised regions were detected by PCR#2 (lower, 1060 bp).

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BMC Biotechnology

ISSN: 1472-6750

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