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Table 2 The primers sequences for RT-PCR and quantitative-PCR assays

From: Engineering an L-cell line that expresses insulin under the control of the glucagon-like peptide-1 promoter for diabetes treatment

Name

Sequences

GLP-rt-F

5' GGC ACA TTC ACC AGC GAC TAC 3'

GLP-rt-R

5' CA ATG GCG ACT TCT TCT GGG 3'

In-rt-F

5' A ACG AGG CTT CTT CTA CAC ACC 3'

In-rt-R

5' TTC CAC AAT GCC ACG CTT CTG 3'

Ac-rt-F

5' GTG TGA TGG TGG GAA TGG GTC 3'

Ac-rt-R

5' AG GAA GAG GAT GCG GCA GTG 3'

B2m-rt-F

5' CTG GTC TTT CTG GTG CTT GTC 3'

B2m-rt-R

5' AT GTG AGG CGG GTG GAA CTG 3'

  1. GLP-rt-F and GLP-rt-R were used to amplify mouse GLP-1 mRNA. In-rt-F and In-rt-R were used to amplify human insulin mRNA. Ac-rt-F and Ac-rt-R were used to amplify mouse β-actin mRNA. B2m-rt-F and B2m-rt-R were used to amplify mouse β-2 microglobulin (β2 m) mRNA.
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BMC Biotechnology

ISSN: 1472-6750

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