Engineering an L-cell line that expresses insulin under the control of the glucagon-like peptide-1 promoter for diabetes treatment

Table 1 The primers sequences for PCR amplification and related restriction enzymes involved

Name	Sequences
LP.Bud-F	5' AT GAG AAA GCT TGT AGA CAG GTG GAG 3'
	Hind III
LP.Bud-R	5' AC AAC ACT AGT GCT TCC AGT CAA ACC 3'
	Spe I
Ins-F	5' AA GTT GTC GAC AGG CTG CAT CAG AAG 3'
	Sal I
Ins-R	5' A TAG GAT CCA CAG GGA CTC CAT CAG 3'
	Bam H I
LP.Blu-F	5' G AAT TCG AGC TGA GAG GAG GTG TAG 3'
	EcoRI
LP.Blu-R	5' C TCG AGA TAC CTG CCT ACC ACT GTC 3'
	XhoI
No-F	5' GA ATT CCA GAA GTA GTG AGG AGG 3'
	EcoR I
No-R	5' T CTA GAT ACA TTG ATG AGT TTG GAC 3'
	Xba I

LP.Bud-F and LP.Bud-R are the primers used for amplification of GLP-1 promoter. Hind III and Spe I are the restriction enzymes used to facilitate GLP-1 promoter cloning into the pBudCE4.1 vector. LP.Blu-F and LP.Blu-R are the primers for amplification of GLP-1 promoter. EcoR I and Xho I are the restriction enzymes used to facilitate GLP-1 promoter cloning into the pBluescript II SK vector. Ins-F and Ins-R are the primers for amplification of insulin gene. Sal I and BamH I are restriction enzymes used to facilitate the cloning of insulin gene into the pBudCE4.1 vector. No-F and No-R are the primers for amplification of neomycin resistant gene. EcoR I and Xba I are the restriction enzymes used to facilitate the cloning of neomycin resistance gene into the pBluescript II SK vector. Underlined sequences are the restriction enzyme sites.

ISSN: 1472-6750