Figure 6From: Engineering an L-cell line that expresses insulin under the control of the glucagon-like peptide-1 promoter for diabetes treatmentThe expression of human insulin protein was analysed using western blotting and immunocytochemistry assays. (A) The result of western blotting shows that mature human insulin was secreted by engineered L-cells. Total protein was extracted from L-1 and L-2. (B) Immunocytochemistry assay confirmed insulin expression in the L-cells. The green sections show that the cytoplasm of the cells expresses human insulin, and the blue sections are nuclei stained by DAPI.Back to article page