Skip to main content

Table 1 Glutamine controls recHCG glycoforms in shake flask cultures.

From: Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Peak
No.
Glycan
Structure
Day 1
0 mM Gln
Day 3
0 mM Gln
Day 5
0 mM Gln
Day 1
4 mM Gln
Day 3
4 mM Gln
Day 5
4 mM Gln
Day 1
8 mM Gln
Day 3
8 mM Gln
Day 5
8 mM Gln
1 A1 15.8 ± 7.4 18.8 ± 3.4 11.5 ± 0.8 7.8 ± 7.6 11.5 ± 3.2 10.0 ± 1.0 6.5 ± 5.8 12.3 ± 3.3 10.8 ± 0.1
2 A2 0.4 ± 0.0 0.7 ± 0.0 0.6 ± 0.1 0.2 ± 0.0 0.4 ± 0.0 0.9 ± 0.1 0.3 ± 0.0 0.5 ± 0.0 0.8 ± 0.0
3 A1G1 2.9 ± 0.4 3.7 ± 0.4 4.2 ± 0.2 0.9 ± 0.4 1.2 ± 0.4 1.6 ± 0.0 1.1 ± 0.2 1.5 ± 0.1 1.7 ± 0.1
4 M5 8.0 ± 0.5 8.0 ± 0.5 8.3 ± 0.4 1.8 ± 0.1 2.8 ± 0.0 3.8 ± 0.0 1.9 ± 0.0 3.3 ± 0.1 4.1 ± 0.1
5 A2G1,
A1G1S1
2.5 ± 0.5 3.0 ± 0.0 3.2 ± 0.3 2.4 ± 0.3 2.6 ± 0.3 3.6 ± 0.3 2.3 ± 0.5 3.0 ± 0.1 3.8 ± 0.0
6 A2G1S1 2.0 ± 0.6 1.8 ± 0.3 2.8 ± 0.1 0.9 ± 0.1 1.5 ± 0.0 1.5 ± 0.1 1.0 ± 0.2 1.3 ± 0.1 1.4 ± 0.1
7 FA2G1 0.8 ± 0.5 1.0 ± 0.0 0.9 ± 0.2 1.2 ± 0.3 1.1 ± 0.1 2.4 ± 0.1 1.2 ± 0.2 1.2 ± 0.1 2.5 ± 0.0
8 A2G2,
FA2G1S1
13.5 ± 1.3 12.6 ± 1.2 16.4 ± 0.1 7.3 ± 0.4 9.3 ± 0.0 8.3 ± 0.5 7.8 ± 0.2 8.5 ± 0.2 7.9 ± 0.4
9 FA2G2,
A2G2S1
20.7 ± 2.0 19.6 ± 1.5 22.6 ± 0.7 22.5 ± 0.5 23.7 ± 0.1 21.3 ± 0.4 23.9 ± 0.1 22.6 ± 0.8 20.3 ± 0.6
10 A2G2S2, FA2G2S1 19.5 ± 1.2 17.1 ± 1.1 17.6 ± 0.4 32.6 ± 5.3 26.1 ± 0.9 26.3 ± 0.2 32.7 ± 4.6 27.1 ± 1.3 25.6 ± 0.4
11 A3G2S1, FA2G2S2 8.8 ± 0.2 9.7 ± 0.7 7.6 ± 0.3 14.6 ± 2.3 12.4 ± 1.6 13.2 ± 1.6 14.1 ± 0.3 12.3 ± 1.1 13.2 ± 0.9
12 A3G2S2,
A3G3S1
1.9 ± 0.5 1.5 ± 0.1 1.7 ± 0.1 2.5 ± 0.2 2.4 ± 0.2 2.2 ± 0.3 2.4 ± 0.3 2.0 ± 0.1 2.5 ± 0.1
13 FA3G3S1, A3G3S2 1.3 ± 0.2 1.0 ± 0.1 1.3 ± 0.1 2.1 ± 0.1 2.1 ± 0.2 1.9 ± 0.1 1.9 ± 0.1 1.8 ± 0.0 2.3 ± 0.0
14 A3G3S3, FA3G3S2 1.1 ± 0.0 0.9 ± 0.1 1.1 ± 0.0 2.3 ± 0.4 1.9 ± 0.2 2.0 ± 0.1 2.2 ± 0.3 1.8 ± 0.1 2.0 ± 0.0
15 FA3G3S3 0.6 ± 0.0 0.5 ± 0.2 0.4 ± 0.1 0.8 ± 0.0 0.9 ± 0.2 1.0 ± 0.2 0.6 ± 0.2 0.8 ± 0.2 1.1 ± 0.0
  1. The relative structures and proportions of each peak of the HCG N-linked glycan NP-HPLC pool and on day 1, 3 and 5 of batch culture with varying concentrations of glutamine. Numbers in bold indicates significant difference, p < 0.05, n = 6.