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Table 1 Glutamine controls recHCG glycoforms in shake flask cultures.

From: Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Peak

No.

Glycan

Structure

Day 1

0 mM Gln

Day 3

0 mM Gln

Day 5

0 mM Gln

Day 1

4 mM Gln

Day 3

4 mM Gln

Day 5

4 mM Gln

Day 1

8 mM Gln

Day 3

8 mM Gln

Day 5

8 mM Gln

1

A1

15.8 ± 7.4

18.8 ± 3.4

11.5 ± 0.8

7.8 ± 7.6

11.5 ± 3.2

10.0 ± 1.0

6.5 ± 5.8

12.3 ± 3.3

10.8 ± 0.1

2

A2

0.4 ± 0.0

0.7 ± 0.0

0.6 ± 0.1

0.2 ± 0.0

0.4 ± 0.0

0.9 ± 0.1

0.3 ± 0.0

0.5 ± 0.0

0.8 ± 0.0

3

A1G1

2.9 ± 0.4

3.7 ± 0.4

4.2 ± 0.2

0.9 ± 0.4

1.2 ± 0.4

1.6 ± 0.0

1.1 ± 0.2

1.5 ± 0.1

1.7 ± 0.1

4

M5

8.0 ± 0.5

8.0 ± 0.5

8.3 ± 0.4

1.8 ± 0.1

2.8 ± 0.0

3.8 ± 0.0

1.9 ± 0.0

3.3 ± 0.1

4.1 ± 0.1

5

A2G1,

A1G1S1

2.5 ± 0.5

3.0 ± 0.0

3.2 ± 0.3

2.4 ± 0.3

2.6 ± 0.3

3.6 ± 0.3

2.3 ± 0.5

3.0 ± 0.1

3.8 ± 0.0

6

A2G1S1

2.0 ± 0.6

1.8 ± 0.3

2.8 ± 0.1

0.9 ± 0.1

1.5 ± 0.0

1.5 ± 0.1

1.0 ± 0.2

1.3 ± 0.1

1.4 ± 0.1

7

FA2G1

0.8 ± 0.5

1.0 ± 0.0

0.9 ± 0.2

1.2 ± 0.3

1.1 ± 0.1

2.4 ± 0.1

1.2 ± 0.2

1.2 ± 0.1

2.5 ± 0.0

8

A2G2,

FA2G1S1

13.5 ± 1.3

12.6 ± 1.2

16.4 ± 0.1

7.3 ± 0.4

9.3 ± 0.0

8.3 ± 0.5

7.8 ± 0.2

8.5 ± 0.2

7.9 ± 0.4

9

FA2G2,

A2G2S1

20.7 ± 2.0

19.6 ± 1.5

22.6 ± 0.7

22.5 ± 0.5

23.7 ± 0.1

21.3 ± 0.4

23.9 ± 0.1

22.6 ± 0.8

20.3 ± 0.6

10

A2G2S2, FA2G2S1

19.5 ± 1.2

17.1 ± 1.1

17.6 ± 0.4

32.6 ± 5.3

26.1 ± 0.9

26.3 ± 0.2

32.7 ± 4.6

27.1 ± 1.3

25.6 ± 0.4

11

A3G2S1, FA2G2S2

8.8 ± 0.2

9.7 ± 0.7

7.6 ± 0.3

14.6 ± 2.3

12.4 ± 1.6

13.2 ± 1.6

14.1 ± 0.3

12.3 ± 1.1

13.2 ± 0.9

12

A3G2S2,

A3G3S1

1.9 ± 0.5

1.5 ± 0.1

1.7 ± 0.1

2.5 ± 0.2

2.4 ± 0.2

2.2 ± 0.3

2.4 ± 0.3

2.0 ± 0.1

2.5 ± 0.1

13

FA3G3S1, A3G3S2

1.3 ± 0.2

1.0 ± 0.1

1.3 ± 0.1

2.1 ± 0.1

2.1 ± 0.2

1.9 ± 0.1

1.9 ± 0.1

1.8 ± 0.0

2.3 ± 0.0

14

A3G3S3, FA3G3S2

1.1 ± 0.0

0.9 ± 0.1

1.1 ± 0.0

2.3 ± 0.4

1.9 ± 0.2

2.0 ± 0.1

2.2 ± 0.3

1.8 ± 0.1

2.0 ± 0.0

15

FA3G3S3

0.6 ± 0.0

0.5 ± 0.2

0.4 ± 0.1

0.8 ± 0.0

0.9 ± 0.2

1.0 ± 0.2

0.6 ± 0.2

0.8 ± 0.2

1.1 ± 0.0

  1. The relative structures and proportions of each peak of the HCG N-linked glycan NP-HPLC pool and on day 1, 3 and 5 of batch culture with varying concentrations of glutamine. Numbers in bold indicates significant difference, p < 0.05, n = 6.