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Figure 10 | BMC Biotechnology

Figure 10

From: Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Figure 10

Sugar nucleotide metabolism. The formation of activated sugar nucleotides is intrinsically linked to cellular metabolism. For example, the conversion of glucose to pyruvate (Pyr) during glycolysis also forms fructose-6-phosphate (Fruc-6-P), which contributes to the accumulation of the UDP-GNAc pool (UDPGlcNAc and UDP-GalNAc), in a similar manner to glutamine (Gln) and ammonium (NH4+). The content of this pool has a direct effect on the proportion of sialic acid precursors (such as CMP-Neu5Ac) and may impact the antennarity of glycans since UDP-GlcNAc is a substrate for GlcNAc transferase enzymes, which catalyse the addition of glycan chains. Glycan donors and monosaccharides are shown in rectangles. Additional abbreviations are noted elsewhere.

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