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Table 1 Primers for PCR of each gene and expected product size

From: Regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9Aa2 gene conferring insect resistance

Target gene

Forward primer (5' to 3')

Reverse primer (5' to 3')

Product size (bp)

cry9Aa2

GCACGGAATTATTGGCGCTTC

CACGATGTCCAACACCATCAA

424

nptII

ATTCGGCTATGACTGGGCACAACA

CCATGATATTCGGCAAGCAGGCAT

536

Actin

GATGGCAGAAGGCGAAGATA

GAGCTGGTCTTTGAAGTCTCG

1069