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Figure 2 | BMC Biotechnology

Figure 2

From: Regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9Aa2 gene conferring insect resistance

Figure 2

PCR analysis of transgenic potato lines. Lanes 1 and 15 are 100 bp molecular ruler N3231 (New England BioLabs) and HyperLadderâ„¢ II (Bioline), respectively; lanes 2-14 represent a multiplexed reaction with the cry9Aa2 primers producing an expected 424 bp product, the nptII primers producing an expected 536 bp product and the actin primers as an internal control producing one or two products depending on endogenous alleles (a 1069 bp product is expected in 'Iwa'); lanes 2, 5, 8 and 11, non-transgenic controls of cultivars 'Iwa', 'Red Rascal', 'Karaka' and ' Pacific', respectively; lanes 3-4 represent transgenic 'Iwa' lines; lanes 6-7 represent transgenic 'Red Rascal' lines; lanes 9-10 represent transgenic 'Karaka' lines; lanes 12-13 represent transgenic 'Pacific' lines; lane 14 is a no-DNA template control.

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