Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco

BMC Biotechnology

Table 1 Accumulation of hG-CSF in tobacco leaves and seeds

Tissue	Copy number¹	Construct	Number of plants²	Average Yield³*	Maxium Yield⁴*
Leaf	single	SH	3	0.21 ± 0.01	0.23
		USH	3	0.42 ± 0.40	0.89
	_	SH	10	0.30 ± 0.07	0.38
		USH	8	0.89 ± 0.77	2.58
Seed	single	SH	3	0.45 ± 0.11	0.53
		USH	3	0.93 ± 0.12	1.07
	_	SH	10	0.50 ± 0.15	0.77
		USH	8	0.97 ± 0.19	1.38

¹, Copy number of trangene determined by Southern blot: single, 1 copy of transgene; -, without consideration to transgene copy number.
², Number of individual transgenic plants anlyzed for each tissue, construct and different copy numbers of transgene.
³, Values are the means ± stand deviation (SD).
⁴, Maxium yield among transgenic plants anlyzed for each construct and tissue.
*, mg/g TSP for leaf and seed, estimated by quatitative analysis of immunoblot data with ImageJ software.

ISSN: 1472-6750