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Table 1 Accumulation of hG-CSF in tobacco leaves and seeds

From: Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco

Tissue Copy number1 Construct Number of plants2 Average Yield3* Maxium Yield4*
Leaf single SH 3 0.21 ± 0.01 0.23
   USH 3 0.42 ± 0.40 0.89
  _ SH 10 0.30 ± 0.07 0.38
   USH 8 0.89 ± 0.77 2.58
Seed single SH 3 0.45 ± 0.11 0.53
   USH 3 0.93 ± 0.12 1.07
  _ SH 10 0.50 ± 0.15 0.77
   USH 8 0.97 ± 0.19 1.38
  1. 1, Copy number of trangene determined by Southern blot: single, 1 copy of transgene; -, without consideration to transgene copy number.
  2. 2, Number of individual transgenic plants anlyzed for each tissue, construct and different copy numbers of transgene.
  3. 3, Values are the means ± stand deviation (SD).
  4. 4, Maxium yield among transgenic plants anlyzed for each construct and tissue.
  5. *, mg/g TSP for leaf and seed, estimated by quatitative analysis of immunoblot data with ImageJ software.