Expression analysis of hG-CSF expressed in tobacco leaves. (A) SDS-PAGE of total soluble protein samples (20 μg/lane) exracted from tobacco leaves. Lanes SH, USH, see Figure 1; WT, wild type tobacco; and M, Precision Plus Protein standards (Bio-Rad). (B) Immunoblot analysis of total soluble protein (20 μg/lane for plant samples, 10-20 ng/lane for comercial hG-CSF as positive control) using anti-hG-CSF antibody. (C) Expression of hG-CSF in individual SH or USH plants in addition to the plants shown in Figure 3 by immunoblot analysis. Arrows marked the location of the molecular weight at 20 and 15 kD. Numbers 4-10 in SH samples and 4-8 in USH samples denoted the individual plants analyzed. Different amounts of Comercial hG-CSF, labled by +, were loaded as postive controls at 5, 10 and 20 ng/lane accordingly.