Figure 4
From: Design and development of a peptide-based adiponectin receptor agonist for cancer treatment
Effects of ADP 355 on intracellular cell signaling in cancer cells. The effects of ADP 355 on signaling pathways in MCF-7, MDA-MB-231, and LN18 cells at 0-60 min of treatment were studied by WB, as described in Methods. The expression of GAPDH was used as determination of protein loading. The relative levels of phosphorylated/total proteins were calculated by densitometry scanning, as described in Methods, and are provided in Additional file 3.