Figure 3
From: Design and development of a peptide-based adiponectin receptor agonist for cancer treatment
Effects of siRNA-mediated downregulation of AdipoR1 or AdipoR2 on ADP 355 activity. The expression of AdipoR1, AdipoR2, and control protein GAPDH were assessed by WB in control (C) cells (transfection medium only), cells treated with scrambled siRNA (Sc siRNA), siRNA targeting AdipoR1, or siRNA targeting AdipoR2, as described in Methods. The relative levels of AdipoR1 and AdipoR2 proteins were calculated by densitometry scanning, as described in Methods, and are provided in Additional file 2. The relative % of growth inhibition upon ADP 355 treatment in cells with different levels of AdipoR1 and AdipoR2 vs. untreated cells is shown in the lower panel table.