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Table 3 Substrate screen for laccase activity and IC decolorization Change of absorbance (ΔA): + (change of absorbance), - (no change of absorbance).

From: Bacillus pumiluslaccase: a heat stable enzyme with a wide substrate spectrum

Structure Compound A IC
R1 = R2 = H 1 - 0
  R1 = H, R2 = OH 2 - 0
  R1 = OH, R2 = H 3 - 0
  R1 = R2 = OH 4 + 0
  R1 = OH, R2 = OCH3 5 + 25
R1 = OH, R2 = R3 = H 6 - 0
  R1 = R2 = OH, R3 = H 7 + 0
  R1 = R2 = R3= OH 8 + 0
  R1 = OH, R2 = R3= OCH3 9 + 0
  R1 = OH, R2 = NH2, R3 = H 10 + 0
  R1 = NH2, R2 = OH, R3 = H 11 + 0
  R1 = OH, R2 = F, R3 = H 12 - 0
R1 = OH, R2 = H 13 - 0
  R1 = OH, R2 = OCH3 14 + 40
  R1 = OCH3, R2 = OH 15 + 0
  16 - 0
R = H 17 - 0
  R = OCH3 18 + >99
R1 = OH, R2 = R3 = H 19 - 0
  R1 = H, R2 = OH, R3 = OCH3 20 + >99
  21 + 0
  22 - 0
R = H 23 - 0
  R = OH 24 + 0
R = H 25 - 0
  R = OH 26 - 0
R1 = R2 = R3 = H 27 - 0
  R1 = R3 = H, R2 = CH3 28 - 0
  R1 = R3 = CH3, R2 = H 29 - 0
  R1 = OH, R2 = R3 = H 30 + 0
  R1 = OH, R2 = CH3, R3 = H 31 + 0
  R1 = R3 = OH, R2 = H 32 + 0
  R1 = R3 = H, R2 = β-O-Gluc 33 - 0
  34 + 0
  35 - 0
  36 - 0
  37 + >99
  1. Indigocarmine decolorization (%) (IC) is given compared to the control reaction lacking laccase.