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Table 2 Half-life of laccase measured by ABTS oxidation.

From: Bacillus pumiluslaccase: a heat stable enzyme with a wide substrate spectrum

  4°C 25°C 45°C 65°C
McIllvaine buffer pH 7 1.0 0.75 0.32 0.28
Potassium phosphate buffer pH 7 0.93 0.79 0.45 0.34
Deionized water 144 40.0 19.3 3.5
  1. The purified protein was stored in different solvents and at various temperatures. Half-lives are given in hours.