Table 1 Kinetic constants of purified laccase from Bacillusspecies for ABTS, 2,6-DMP and SGZ oxidation.
From: Bacillus pumiluslaccase: a heat stable enzyme with a wide substrate spectrum
Substrate | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Laccases | ABTS | 2, 6-DMP | SGZ | ||||||
|  | K M (μM) | kcat (s-1) | Assay conditions | KM (μM) | kcat (s-1) | Assay conditions | KM (μM) | k cat (s-1) | Assay conditions |
B. pumilus | 80 ± 4 | 291 ± 2.7 | 0.6 μM-10 mM, pH 4, RT | 680 ± 27 | 11 ± 0.1 | 0.6 μM-10 mM, pH 7, RT | n.d. | *4.9 | 6 - 78 μM, <0.8% (v/v) DMSO pH 6.5, RT |
|  |  |  |  |  |  |  | n.d. | *66 ± 1.5 | 0.04 μM-1.25 mM (25% (v/v) DMSO), pH 7, RT |
B. subtilis [26] | 124 ± 17 | 322 ± 20 | 10 -200 μM pH 4, 37°C | 216 ± 35 | 29 ± 4 | 10 -200 μM, pH 7, 37°C | 18 ± 3 | 80 ± 4 | 1 -100 μM, pH 7, 37°C |
B. licheniformis [25] | 6.5 ± 0.2 | 83 | 5 -500 μM pH 4, RT | 56.7 ± 1 | 28 | 5 -1000 μM, pH 7, RT | 4.3 ± 0.2 | 100 | 1.25 -50 μM, pH 7, RT |