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Table 1 Kinetic constants of purified laccase from Bacillusspecies for ABTS, 2,6-DMP and SGZ oxidation.

From: Bacillus pumiluslaccase: a heat stable enzyme with a wide substrate spectrum

Substrate
Laccases ABTS 2, 6-DMP SGZ
  K M (μM) kcat (s-1) Assay conditions KM (μM) kcat (s-1) Assay conditions KM (μM) k cat (s-1) Assay conditions
B. pumilus 80 ± 4 291 ± 2.7 0.6 μM-10 mM, pH 4, RT 680 ± 27 11 ± 0.1 0.6 μM-10 mM, pH 7, RT n.d. *4.9 6 - 78 μM, <0.8% (v/v) DMSO pH 6.5, RT
        n.d. *66 ± 1.5 0.04 μM-1.25 mM (25% (v/v) DMSO), pH 7, RT
B. subtilis [26] 124 ± 17 322 ± 20 10 -200 μM pH 4, 37°C 216 ± 35 29 ± 4 10 -200 μM, pH 7, 37°C 18 ± 3 80 ± 4 1 -100 μM, pH 7, 37°C
B. licheniformis [25] 6.5 ± 0.2 83 5 -500 μM pH 4, RT 56.7 ± 1 28 5 -1000 μM, pH 7, RT 4.3 ± 0.2 100 1.25 -50 μM, pH 7, RT
  1. Substrate ranges covered, pH and temperature are listed. n.m.: not measurable, due to substrate solubility. * The value was deduced from the highest observed rate.