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Table 1 Fold changes of CYP450 isotypes' expression in three cell types after 72 h of induction with prototypic CYP450 inducers (omeprazole, dexamethasone, rifampicin, artemisinine and ethanol) over the untreated control

From: Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers

P450 isotypes/enzyme inducers concentration (μM) mRNA fold change (mean ± SD)
   HepG2 P-hepatocyte hMSC Hep-like cell
CYP1A1      
omeprazole 50 4.05 ± 0.38* 11.13 ± 0.34* 2.09 ± 0.30 2.13 ± 0.15
dexamethasone 25 1.11 ± 0.13 19.68 ± 0.46* 3.10 ± 0.33* 12.86 ± 0.39*
CYP1A2      
omeprazole 50 3.33 ± 0.14* 6.44 ± 0.80 1.67 ± 0.12 2.37 ± 0.13
dexamethasone 25 1.17 ± 0.02 13.62 ± 2.84* 3.82 ± 0.96* 5.63 ± 0.65*
CYP2B6      
rifampicin 40 25.82 ± 1.30* 35.16 ± 2.76** 1.46 ± 0.10 30.70 ± 5.36**
dexamethasone 25 2.58 ± 0.72 41.49 ± 4.58** 3.98 ± 0.71* 24.25 ± 2.30*
CYP2D6      
dexamethasone 25 1.26 ± 0.10 5.70 ± 1.29 2.68 ± 0.43 2.90 ± 0.73
CYP2C9      
rifampicin 40 1.42 ± 0.25 15.25 ± 2.51* 2.03 ± 0.22 7.78 ± 1.82*
CYP2C19      
rifampicin 40 1.87 ± 0.11 29.05 ± 2.02** 2.42 ± 0.61 19.01 ± 2.51*
CYP2C8      
rifampicin 40 9.07 ± 1.03* 24.40 ± 2.48* 3.03 ± 0.62* 12.28 ± 0.81*
CYP3A4      
rifampicin 40 39.43 ± 5.53** 69.50 ± 6.84** 3.64 ± 1.82* 43.29 ± 3.27**
dexamethasone 25 23.18 ± 7.25** 72.27 ± 5.64** 2.87 ± 0.96 84.10 ± 9.25**
artemisinine 50 48.18 ± 6.80** 59.28 ± 8.41** 0.89 ± 0.07 53.54 ± 1.37**
CYP2E1      
ethanol 88 4.59 ± 0.53* 24.23 ± 0.41* 1.46 ± 0.19 10.74 ± 2.10*
  1. hMSC, HepG2, primary hepatocyte and hepatocyte-like cell were induced with prototypic inducer drugs. The CYP450 mRNA level was detected by Real-time PCR. The experiment was performed in triplicate for all compounds. Data was normalized by untreated (non-treated controls) and was analyzed by Non parametric One-tailed Student t test (Mann-Whitney test) * p < 0.05, ** p < 0.01.