TLR9 activation by B lymphocytes, PBMC, and CAL-1 cells. (A) Activation of NF-κB in human B lymphocyte cell lines, Ramos-Blue cells, stably transfected with NF-kB/AP-1-inducible SEAP reporter gene. n = 3, mean (sd). All P values are shown relative to the PD-ODN2006: *P < 0.05, **P < 0.01. (B-C) IL-6 production induced by CpG-ODNs (1: PD-ODN2006, 2: PD-ODN2006-2006, 3: PD-ODN2006-2006-2006, 4: PD-ODN2006-G5, 5: PTO-ODN2006). n = 3, mean (sd). From human PBMCs (B) and human plasmacytoid dendritic cells CAL-1(C). The symbol # indicates that no cytokine secretion was detected.