Figure 2
Effect of terminal modification and linker sequences in PD-ODN2006 on NF-κB activation. (A) 293XL-hTLR9 cells transfected with hTLR9-signaling reporter plasmid were stimulated by modified PD-ODN2006s (1: PD-ODN2006, 2: PD-ODN-3'-Biotin, 3: PD-ODN-5'-Biotin, 4: PD-ODN-3'-FITC, 5: PD-ODN-5'-FITC, 6: PD-ODN-3'-NH2, 7: PD-ODN-5'-NH2, 8: PD-ODN-3'-A, 9: PD-ODN-5'-A, 10: PD-ODN-3'-B, 11: PD-ODN-5'-B) at a concentration of 0.5 μM. n = 3, mean (sd). All P values are shown relative to the PD-ODN2006, *P < 0.05, **P < 0.01. (B) Stability of modified PD-ODN2006s in aqueous solution with/without serum. (C) 293XL-hTLR9 cells transfected with hTLR9-signaling reporter plasmid were stimulated by series connection of PD-ODN2006 with/without linker sequences. n = 3, mean (sd). (D) Stability of these PD-ODNs in aqueous solution with/without serum.