Figure 2From: Directed PCR-free engineering of highly repetitive DNA sequencesGeneration of long repetitive sequences can be accelerated. The elongation of the Q-encoding region was accelerated by a simple modification of the cloning strategy. For example, the Poly-Q encoding region of the plasmid pMK1-Q20 can be excised with BsaI and SacI and ligated into the same vector digested with BsmBI/SacI. Thereby, the number of Q-encoding repeats can be almost doubled in a single cloning step.Back to article page