Figure 10

SEB detection using Luminex sandwich assays. Three different anti-SEB antibody coated bead sets plus a negative control were used in each assay performed as described in the methods. Top panel used Bt-sdAb-A3 (1 μg/mL), middle panel used Bt-MAb 3b2a (10 μg/mL), and bottom panel Bt-Llama anti-SEB (10 μg/mL), respectively as the detector antibody, followed by SA-PE (5 μg/mL) to fluorescently label the immuno-complex prior to measurement on the Luminex 100.