Multiple sequence alignment of activated Cry toxins after digestion (mature toxin) including translated Cry8Ka1 and mutant Cry8Ka5 sequences. From top to bottom the sequences are Cry2Aa (Uniprot code: P0A377), Cry4Ba (P05519), Cry4Aa (P16480), Cry1Aa (BAA04468), Cry 3Aa (P0A379), Cry3Bb1 (Q06117), Cry8Ea1 (NCBI accession: AAQ73470.1), Cry8Ka1 (FJ422558) e Cry8Ka5. The secondary structure of Cry8Ka1 is indicated in the bottom line. The Cry8Ka α-helix colors match up to the same α-helix colors presented in our homology model (Fig 6) constructed using Cry8Ea1 (PDB entry 3EB7) as template. The highly conserved boxes of Cry family are indicated by gray color and labeled in the top line. Highly conserved residues are highlighted in green. The five replaced residues in the Cry8Ka5 sequence comparing to Cry8Ka1 are highlighted in magenta. The numbers in the bottom line are those of the structural Cry8Ka1 model.