Graphic representation indicates number of substitutions in the nucleotide sequences, distributed among the three domains for that seven selected cry8Ka1 variants. The sequences of variants named cry8Ka2, cry8Ka3, cry8Ka4, cry8Ka 5, cry8Ka6, cry8Ka7 and cryKa8 were fully sequenced and aligned using cry8Ka1. In black color, indicate domain I, in gray color, domain II and white color, domain III. In each variant a large number of nucleotide mutations was observed. The cry8Ka5 variant contained 578 nucleotide substitutions in total.