Figure 3

SDS-PAGE electrophoresis and Western blot analysis of Cry8Ka1 and Cry8Ka5 expressed in E. coli cells. The original and variant recombinant Cry8K toxins were fractionated by SDS-PAGE, blotted onto a nitrocellulose membrane and probed with monoclonal Anti-His^® antibody A) Lane 1, BenchMark™ Protein Ladder (Invitrogen); lane 2, proteins from non-induced cells (supernatant); lane 3, proteins expressed after induction with 0.5 mM IPTG-(supernatant); lane 4 and 5, Cry8Ka5 and Cry8Ka1 recombinant toxins, respectively, purified by using affinity chromatography as described in material and methods. B) Western immunoblot analysis. Lane 1 and 2 the arrows indicate Cry8Ka5 and Cry8Ka1 recombinant toxins, both with approximate molecular weights of 70 kDa.