Figure 1From: Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)Illustration of the DNA shuffling steps. The DNA shuffling product was analyzed on 2.5% agarose gel electrophoresis. Lane M: 1Kb-plus ladder (Invitrogen); lane 1: cry8Ka1 gene amplification containing only the region correspondent to mature toxin (Domains 1, II and III - 2001 bp); lane 2: DNAse I digestion product resulting to fragments of 50 bp; lane 3: Reassembled PCR product using as template fragments containing 50 bp (obtained jointly and gel purified) and no primers added to reaction; lane 4: PCR amplification containing pool of variants (around 2000 bp) to reassembled genes.Back to article page