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Table 4 Influenza infectious particle production in batch and perfusion cultures at different days post-infection (dpi).

From: Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

  TCID50 titers, TCID50 units/mL Total productivity, TCID50 units Specific productivity, TCID50 units /103 cell
  1 dpi 2 dpi 3 dpi 4 dpi 1 dpi 2 dpi 3 dpi 4 dpi 1 dpi 2 dpi 3 dpi 4 dpi
BATCH 3.0 × 109 5.3 × 1010 3.0 × 108   1.0 × 1013 1.6 × 1014 7.9 × 1011   0.7 10.4 0.1  
PERFUSION 2.0 × 109 2.1 × 1010 3.3 × 1011 2.0 × 109 7.9 × 1012 1.6 × 1014 1.0 × 1015 2.5 × 1014 0.3 4.9 36.8 8.5
  1. Total productivity was calculated at different times of harvest post-infection (Harvest of the perfused flow, 1.5 L/day, was taken into account in the calculation for total productivity in the perfusion system). Specific productivities were calculated based on total productivity and maximum cell density attained (5.76 × 106 cells/mL in batch and 10.1 × 106 cells/mL in the perfusion system).