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Table 4 Influenza infectious particle production in batch and perfusion cultures at different days post-infection (dpi).

From: Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

 

TCID50 titers, TCID50 units/mL

Total productivity, TCID50 units

Specific productivity, TCID50 units /103 cell

 

1 dpi

2 dpi

3 dpi

4 dpi

1 dpi

2 dpi

3 dpi

4 dpi

1 dpi

2 dpi

3 dpi

4 dpi

BATCH

3.0 × 109

5.3 × 1010

3.0 × 108

 

1.0 × 1013

1.6 × 1014

7.9 × 1011

 

0.7

10.4

0.1

 

PERFUSION

2.0 × 109

2.1 × 1010

3.3 × 1011

2.0 × 109

7.9 × 1012

1.6 × 1014

1.0 × 1015

2.5 × 1014

0.3

4.9

36.8

8.5

  1. Total productivity was calculated at different times of harvest post-infection (Harvest of the perfused flow, 1.5 L/day, was taken into account in the calculation for total productivity in the perfusion system). Specific productivities were calculated based on total productivity and maximum cell density attained (5.76 × 106 cells/mL in batch and 10.1 × 106 cells/mL in the perfusion system).
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BMC Biotechnology

ISSN: 1472-6750

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