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Table 3 Influenza HA production in batch and perfusion culture at different days post-infection (dpi).

From: Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

  HA titers, logHA units/mL Total productivity, logHA units Specific productivity, HA units/105 cell
  1 dpi 2 dpi 3 dpi 4 dpi 1 dpi 2 dpi 3 dpi 4 dpi 1 dpi 2 dpi 3 dpi 4 dpi
BATCH 2.5 3.7 3.4   6.0 7.2 6.9   6 97 49  
PERFUSION 1.9 4.3 4.0 3.8 5.4 7.8 7.8 7.7 0.9 199 191 173
  1. Total viral particles were quantified by hemaglutination assays (HA titers). Total productivity was calculated at different times of harvest post-infection (Harvest of the perfused flow, 1.5 L/day, was taken into account in the calculation for perfusion system). Specific productivities were calculated based on total productivity and the maximum cell density attained (5.76 × 106 cell/mL in batch and 10.1 × 106 cell/mL in the perfusion system).