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Table 1 Impact of feeding mode, batch or perfusion, and of influenza infection on HEK293SF cell growth and metabolism

From: Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

  Standard
deviation
Batch
culture
Infected batch culture Perfusion
culture
Infected perfusion culture
    pre-infection post-infection   pre-infection post-infection
μ max , h-1 10% 0.021    0.02 -0.013 0.024    0.023 0.019
Y Glc/X , mmol.10-6cell 17% 0.023    0.023 0.026 0.056    0.057 0.038
Y Lact/X , mmol.10-6cell 22% 0.012    0.014 0.01 0.088    0.083 0.037
Y Gln/X , mmol.10-6cell 10% 0.007    0.007 0.001 0.018    0.014 0.01
Y Amm/X , mmol.10-6cell 43% 0.001    0.001 0.002 0.003    0.003 0.009
Y Lact/Glc , mol.mol-1 19% 0.5    0.6 0.4 1.6    1.5 1 .0
Y Amm/Gln , mol.mol-1 43% 0.1    0.1 2 .0 0.2    0.3 1.1
  1. The values presented in bold letters correspond to culture phases with infection with A/PR/8/34 influenza strain.