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Figure 1 | BMC Biotechnology

Figure 1

From: Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism

Figure 1

Schematic representation of mismatch 'bubbles' created by annealing template and mismatch-inducing oligonucleotides. Mismatches are specifically placed surrounding the mutation site to produce differential binding to the wild-type and mutation templates based solely on the identity of the template. By modifying the position and number of mismatches, different size mismatch 'bubbles' are generated, which may become sensitive to nuclease treatment if sufficient ssDNA is exposed. Here, a stepwise increase in mismatches is explored to determine the minimal 'bubble' size that can confer nuclease sensitivity to the mutant template while maintaining resistance by the wild-type template.

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