Application of rhPCR to determine genotype for 31 individuals at the SMAD7 rs4939827 SNP Locus. Human DNA samples from 31 individuals comprising 10 C/C homozygotes (circles), 10 T/T homozygotes (squares), and 11 C/T heterozygotes (triangles) were obtained from the Coriell Cell Repository and genotypes were determined using rhPCR. Reactions were run in triplicate using 2 ng of genomic DNA with SYBR® Green detection. Separate reactions using unmodified non-discriminatory control primers were performed to ensure that the amount of template was approximately equal in each case. A. Reactions were run in real-time mode for 45 cycles. Cq values for triplicate reactions were averaged and plotted according to the primer employed (rC vs. rU). B. Reactions were run in end-point mode for 35 cycles. The raw fluorescence measurements from each reaction are shown and plotted according to the primer employed (rC vs. rU).